PE-22-28_RUO 10mg

PE-22-28 10mg is a synthetic heptapeptide (7-amino-acid) analog of spadin (a naturally occurring peptide fragment from the sortilin propeptide), optimized as a potent and selective inhibitor of the TREK-1 (TWIK-related K⁺ channel 1, also known as KCNK2) two-pore domain potassium channel. With an reported IC₅₀ of approximately 0.12 nM, this shortened sequence exhibits enhanced potency, stability, and specificity compared to the parent spadin (PE 12-28). The sequence is Gly-Val-Ser-Trp-Gly-Leu-Arg (GVSWGLR), with a molecular formula of C₃₅H₅₅N₁₁O₉ and a molecular weight of approximately 773.88–773.9 Da (variations noted across sources due to exact form or acetate salt). Supplied as high-purity lyophilized powder (>98% purity, verified by HPLC and mass spectrometry), PE-22-28 is intended exclusively for in vitro research and scientific experimentation. It is commonly utilized in preclinical laboratory models to investigate TREK-1 channel blockade mechanisms, modulation of neuronal excitability, serotonergic signaling enhancement (e.g., increased firing in dorsal raphe nucleus neurons), neuroplasticity pathways (including upregulation of BDNF/TrkB signaling, PSD-95 expression, and synaptogenesis markers), neurogenesis promotion in hippocampal regions, anti-apoptotic effects under ischemic or stress conditions, and related processes in cellular assays (e.g., HEK293 or neuronal cultures), tissue models, or animal systems exploring mood regulation, depression models, stroke recovery, cognitive dynamics, neurodegeneration, and stress resilience.